Regulation of lipoprotein lipase-mediated lipolysis of triglycerides

Effects of sphingomyelin and cholesterol on lipoprotein lipase-mediated lipolysis in lipid emulsions.

Lipoprotein lipase (LPL) hydrolyzes triacylglycerol (TG) of TG-rich lipoproteins. We investigated the effects of sphingomyelin (SM) and cholesterol (Chol) on the lipolysis of lipid emulsions by LPL using human apolipoprotein C-II (apoC-II) or plasma as an activator. Kinetic studies of the lipolysis rates clearly demonstrated that the primary effect of the activator on the LPL reaction was not t...

Ritonavir impairs lipoprotein lipase-mediated lipolysis and decreases uptake of fatty acids in adipose tissue.

OBJECTIVE The use of the HIV protease inhibitor ritonavir (RTV) is frequently associated with hypertriglyceridemia and lipodystrophy. The aim of our study was to determine the mechanism underlying the observed hypertriglyceridemia. METHODS AND RESULTS Feeding female APOE*3-Leiden transgenic mice a Western-type diet supplemented with RTV (35 mg/kg per day) for 2 weeks resulted in a 2-fold incr...

The role of clearing-factor lipase (lipoprotein lipase) in the transport of plasma triglycerides.

The enzyme clearing-factor lipase, or lipoprotein lipase, is responsible for the removal of most of the plasma triglycerides from the bloodstream (Robinson, 1963). The main carriers of such triglycerides are the chylomicrons and the verylowdensity lipoproteins and these complexes are believed to be first sequestered by the enzyme at the lumen surfaces of the capillary endothelial cells of the e...

Studies of fat lipolysis by post-heparin human plasma lipoprotein lipase and by human pancreatic lipase.

Lipoprotein lipase and lipolysis: central roles in lipoprotein metabolism and atherogenesis.

Although it has been known for over 50 years that lipoprotein lipase (LPL) hydrolyzes triglyceride in chylomicrons, during the past half decade there has been a reinterest in the physiologic and pathophysiologic actions of this enzyme. In part, this has coincided with clinical studies implicating increased postprandial lipemia as a risk factor for atherosclerosis development. In addition, the r...